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Center of Excellence in Environmental Studies
Document Details
Document Type
:
Article In Journal
Document Title
:
Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate
تأثير الرطوبة النسبية على الشكل الظاهرى لنقط مطبوعة بحبر على مواد غير مثقبة
Subject
:
Enviromental sciences
Document Language
:
English
Abstract
:
During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain” spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e., <60%), a spot morphology resembling a coffee stain was prominent, whereas a more homogeneous distribution was observed when droplets were printed and dried at a higher relative humidity (70%).
ISSN
:
1473-7604
Journal Name
:
The Royal Society of Chemistry
Volume
:
0
Issue Number
:
0
Publishing Year
:
1435 AH
2014 AD
Article Type
:
Article
Added Date
:
Monday, May 26, 2014
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
Liyakat Hamid Mujawar
Mujawar, Liyakat Hamid
Researcher
Willem.Norde@wur.nl
J. G. M. Kuerten
Kuerten, J. G. M.
Researcher
daniel.siregar@gmail.com
D. P. Siregar
Siregar, D. P.
Researcher
daniel.siregar@gmail.com
Aart van Amerongen
Amerongen, Aart van
Researcher
Aart.vanamerongen@wur.nl
Willem Norde
Norde, Willem
Researcher
Willem.Norde@wur.nl
Files
File Name
Type
Description
36993.pdf
pdf
Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate
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